Journal: CNS neuroscience & therapeutics
Article Title: Alleviation of Microglia Mediating Hippocampal Neuron Impairments and Depression-Related Behaviors by Urolithin B via the SIRT1-FOXO1 Pathway.
doi: 10.1111/cns.70379
Figure Lengend Snippet: FIGURE 2 | UB treatment ameliorates depression-like behaviors and neuroinflammation and abnormal expression of SIRT1 and FOXO1 in the hippocampus of LPS-exposed mice. (A) Scheme of the experimental procedure. (B) SPT, FST, and locomotor tests. (C) Relative mRNA levels of TNF-ɑ, IL-1ɑ, IL-1β, IL-6, and IDO in the hippocampus. mRNA levels of M1 type markers iNOS and CD86 (D), and M2 type markers ARG1 and CD206 (E) in the hippocampus. (F and G) Western blot was performed to evaluate the effect of UB on the protein levels of Iba1, IDO, SIRT1, and FOXO1 in the hippocampus. n = 6–7 per group. #p < 0.05, ##p < 0.01, ###p < 0.001 versus the Vehicle + Vehicle group or LPS + Vehicle group.
Article Snippet: The primary antibodies employed in this study were as follows: rabbit anti- SIRT1 (Sigma- Aldrich, 07- 131), rabbit anti- FOXO1 (CST, 2880S), mouse anti- β- actin (CST, 3700S), rabbit anti- P65 (CST, 8242T), rabbit anti- phospho- P65 (CST, 3031S), ARG1 (BOSTER, A01106), CD206 (BOSTER, A02285- 2), CD86 (BOSTER, A00220- 4), iNOS (BOSTER, BA0362), ERK (CST, 4695), p- ERK (CST, 4370), rabbit anti- P38 (CST, 9212S), phospho- P38 (CST, 9215), JNK (CST, 9251), phospho- JNK (CST, 9252), rabbit anti- AMPK (CST, 5831S), rabbit anti- phospho- AMPK (CST, 2535S), rabbit anti- Bax (CST, 5023T), mouse anti- Bcl- 2 (CST, 15071T), mouse anti- IDO (Proteintech, 66528- 1- Ig), rabbit anti- Ac- FOXO1 (Invitrogen, PA5- 104560), rabbit anti- Ac- P65 (Abcam, AB19870).
Techniques: Expressing, Western Blot